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mrc 5 cell lines  (ATCC)


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    ATCC mrc 5 cell lines
    Mrc 5 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5458 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mrc+5+cells/MRC-5/pm42302327-215-1-21
    Average 99 stars, based on 5458 article reviews
    mrc 5 cell lines - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: SARS-CoV-2 spike protein exerts an anti-cancer effect in A549 cells in association with MEG3 and BCYRN1 regulation.
    Article Snippet: A549 cells (Cat: CCL-185, ATCC, Manassas, VA, USA) were cultured in RPMI 1640 (Cat: LM 011-01, WELGENE, Daegu, Korea) supplemented with 10% fetal bovine serum (FBS) (Cat: S101-07, WELGENE, Daegu, Korea) and 1% antibiotics (Cat: LS203-01, WELGENE, Daegu, Korea). .. Lenti-X 293T cells (Cat: 632180, ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS Takara Bio Inc., Shiga, Japan) and MRC-5 cells (Cat: CCL-1171 ATCC, Manassas, VA, USA) were cultured in DMEM (Cat: LM-001-05, WELGENE, Daegu, Korea) supplemented with 10% FBS and 1% antibiotics. ..

    Article Title: Evaluating Spike Antigenicity across Endemic Human Coronavirus Models using Flow Virometry
    Article Snippet: A549 (BEI Resources, Cat #NR-52268), Vero E6 cells (from Dr. Phyllis Kanki) and Huh7.5 cells (provided by Deborah R. Taylor, US Food and Drug Administration, RRID: CVCL_7927) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, Cat# 10313-021). .. MRC-5 cells were cultured in EMEM (ATCC, Cat# 30-2003) supplemented with Antibiotic-Antimycotic (Gibco, Cat# 15240062). .. HCT-8 (HRT-18) cells (ATCC, Cat# CCL-244) were cultured in RPMI (Gibco, Cat#11875-093).

    Virus:

    Article Title: Development and Validation of a Quantitative RT-qPCR Panel for the Detection and Monitoring of Polioviruses in Wastewater Samples.
    Article Snippet: .. Human coronavirus 229E (HCoV-229E) was purchased from the ATCC (VR-740) and propagated in MRC-5 cells, and aliquots of the virus stock were stored at −80 ◦C. ..

    Infection:

    Article Title: Resolving in vitro heterogeneity of host functional responses to HCoV-229E via single-cell analyses
    Article Snippet: .. Several aliquots of 10 6 MRC-5 cells were cryopreserved for infection experiments at passage #2 from the original source vial using ice-cold EMEM supplemented with 25% (v/v) filter-sterilized FBS and 5% (v/v) Dimethyl Sulfoxide (DMSO: ATCC, #4-X). .. Human coronavirus strain 229E (HCoV-229E) from ATCC ((#VR-740, Lot #70053995) was used for in-house virus batch propagation.



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    (A) Supernatants from HCoV-229E-infected human cell lines (MRC-5, Huh7.5, and HCT-8; top to bottom rows) were stained using S-specific mAbs targeting the RBD (C04), S1 (F07), or S2 (F12) domains, or an isotype control (IgG), followed by a PE-labeled secondary antibody. Gates for positive staining were set above the isotype control background (>15 PE MESF on the y-axis). Calibrated mean PE fluorescence values (MESF) from gated regions are shown in red. (B) Quantitative comparison of staining results from the gates in (A), with bars representing mean PE MESF ± standard deviation wherein light blue = MRC-5, blue = Huh7.5, and dark blue = HCT-8. Data are representative of at least three independent replicates. Statistical significance was determined using two-way ANOVA with Dunnett’s multiple comparisons test to compare each antibody condition to the IgG control within each virus. Significance thresholds: **p < 0.01, ***p < 0.001, ****p < 0.0001.(C) HCoV-229E pseudoviruses produced by transfection of 293T cells were stained directly in cell culture supernatants using the same antibody panel and staining protocol as in (A). (D) Quantification of staining in (C), with bars indicating mean PE MESF ± standard deviation. See also Figures S1 - S3.

    Journal: bioRxiv

    Article Title: Evaluating Spike Antigenicity across Endemic Human Coronavirus Models using Flow Virometry

    doi: 10.64898/2026.05.28.728498

    Figure Lengend Snippet: (A) Supernatants from HCoV-229E-infected human cell lines (MRC-5, Huh7.5, and HCT-8; top to bottom rows) were stained using S-specific mAbs targeting the RBD (C04), S1 (F07), or S2 (F12) domains, or an isotype control (IgG), followed by a PE-labeled secondary antibody. Gates for positive staining were set above the isotype control background (>15 PE MESF on the y-axis). Calibrated mean PE fluorescence values (MESF) from gated regions are shown in red. (B) Quantitative comparison of staining results from the gates in (A), with bars representing mean PE MESF ± standard deviation wherein light blue = MRC-5, blue = Huh7.5, and dark blue = HCT-8. Data are representative of at least three independent replicates. Statistical significance was determined using two-way ANOVA with Dunnett’s multiple comparisons test to compare each antibody condition to the IgG control within each virus. Significance thresholds: **p < 0.01, ***p < 0.001, ****p < 0.0001.(C) HCoV-229E pseudoviruses produced by transfection of 293T cells were stained directly in cell culture supernatants using the same antibody panel and staining protocol as in (A). (D) Quantification of staining in (C), with bars indicating mean PE MESF ± standard deviation. See also Figures S1 - S3.

    Article Snippet: MRC-5 cells were cultured in EMEM (ATCC, Cat# 30-2003) supplemented with Antibiotic-Antimycotic (Gibco, Cat# 15240062).

    Techniques: Infection, Staining, Control, Labeling, Fluorescence, Comparison, Standard Deviation, Virus, Produced, Transfection, Cell Culture

    (A) Binding of mAbs to recombinant S-2P as determined by ELISA. Data represent at least two independent experiments, each with triplicate wells. Data are representative of two independent experiments with staining performed in duplicate wells. (B) Replication-competent HCoV-229E and HCoV-OC43 grown in MRC-5 cells, HEK293T-derived MERS-CoV pseudoviruses, and SARS-CoV-2 propagated in A549-ACE2 cells, were stained with a panel of S2-specific mAbs and analyzed by flow virometry. Gates represent total antibody-stained virus populations relative to the isotype control (IgG). (C) Quantitative comparison of staining results from the gates in (B). See also Figure S5.

    Journal: bioRxiv

    Article Title: Evaluating Spike Antigenicity across Endemic Human Coronavirus Models using Flow Virometry

    doi: 10.64898/2026.05.28.728498

    Figure Lengend Snippet: (A) Binding of mAbs to recombinant S-2P as determined by ELISA. Data represent at least two independent experiments, each with triplicate wells. Data are representative of two independent experiments with staining performed in duplicate wells. (B) Replication-competent HCoV-229E and HCoV-OC43 grown in MRC-5 cells, HEK293T-derived MERS-CoV pseudoviruses, and SARS-CoV-2 propagated in A549-ACE2 cells, were stained with a panel of S2-specific mAbs and analyzed by flow virometry. Gates represent total antibody-stained virus populations relative to the isotype control (IgG). (C) Quantitative comparison of staining results from the gates in (B). See also Figure S5.

    Article Snippet: MRC-5 cells were cultured in EMEM (ATCC, Cat# 30-2003) supplemented with Antibiotic-Antimycotic (Gibco, Cat# 15240062).

    Techniques: Binding Assay, Recombinant, Enzyme-linked Immunosorbent Assay, Staining, Derivative Assay, Virus, Control, Comparison